Fig. 1

MG132 inhibited the proliferation of ACC-83 cells. A ACC-83 cells were treated with MG132 (0, 2.5, 5, 10, 40, 70, 100µM) for 12 h. B EC50 = 41.68µM was calculated by GraphPad Prism. C. Cells were treated with 40µM MG132 for 3, 6, 9, 12, 24, 36 h. The final concentrations of the compounds were as shown. Cell viability was determined by CCK8 assay (mean ± SD). All data was obtained from ten independent experiments performed in triplicate. *P < 0.05,**P < 0.01,***P < 0.001 versus control