Fig. 7
Upper panel [A]: showing representative sample for i-PRF group showing CBCT analysis and photomicrographs of dog’s tooth treated with blood clot scaffold (a) Digital post-operative radiograph showing a periapical lesion. (b) Horizontal view of CBCT image showing pulp space with intracanal calcific tissues “white arrow” and a periapical lesion “red arrow”. (c&c*) Horizontal cuts of CBCT image showing middle and apical thirds of the root, respectively, notice the presence of intracanal calcific tissues. (d) Photomicrograph showing periapical inflammation and mineralized tissue formation up to cervical third (e&f) Coronal and middle thirds, respectively, at higher magnification, showing bone-like “black arrow heads”, cementum-like “red arrow heads”. (g) Apical third showing periodontal-like tissue formation “green arrow heads”, notice the root resorption and inflammatory infiltrate at the periapical area. (h) Bridge formation under the MTA coronal plug (H&E: d, e, f, g&h); (10x: d; 40x: e, f&g; 100x: h). Lower panel [B]: showing representative sample for HA group showing CBCT analysis and photomicrographs of dog’s tooth treated with HA scaffold (i) Digital post-operative radiograph showing small periapical lesion. (j) Coronal view of CBCT image showing pulp space with minimal intracanal calcific tissues at apical third and a small sized periapical lesion. (k) Sagittal view of CBCT image showing pulp space with minimal intracanal calcific tissues at apical third and a small sized periapical lesion (l&l*) Horizontal cuts of CBCT image showing coronal and apical thirds of the root, respectively, notice the presence of intracanal calcific tissues only in apical third. (m) Photomicrograph showing periapical healing and tissue formation only at apical third (n) Showing periapical healing with no inflammatory cells and new tissue formation at the apical third of canal lumen. (o) Higher magnification of (m) showing cementum-like tissue “black arrow heads”, periodontal ligament PDL -like tissue “green arrow heads” and new blood vessels “red arrow heads”. (H&E: m, n &o); (10x: m; 40x: n; 100x: o)