Fig. 8
Representative sample for P-ECM group showing CBCT analysis and photomicrographs of dog’s tooth treated with P-ECM scaffold (a) Digital preoperative radiograph showing normal periapical condition. (b) Digital post-operative radiograph showing periapical lesion (c) Coronal view of CBCT image showing pulp space with minimal intracanal calcific tissues at apical third “white arrow” and a periapical lesion “red arrow”. (d&d*) Horizontal cuts of CBCT image showing middle and apical thirds of the root, respectively, Notice the presence of intracanal calcific tissues in the apical third. (e&f) Photomicrographs showing periapical healing and regenerated tissue up to the level of MTA coronal plug. (g) Apical third of canal showing periapical cementum healing and ingrowth of regenerated tissues (h) Middle third of canal showing vascularized soft tissue and mineralized tissue formation. (i) Same sample showing numerous blood vessels “black arrow heads”, dense connective tissue fibers (j, k&l) Higher magnification showing cementum-like tissue with cells lining the inner canal wall “black arrow heads”, dense connective tissue fibers “green arrow heads” and new blood vessels “red arrow heads”. (H&E: e, g,h, j,k&l); (MT: f&i); (10x: e&f; 100x: g,h&i; 400x: j,k&l)