Fig. 4

AREG enhances the immunosuppressive effect of Tregs. CD4⁺CD25^hiCD127^lo T cells were sorted by magnetic cell separation from PBMCs and were cocultured with CD4⁺ T cells labeled with CFSE for 3 days in the presence or absence of 100 ng/ml recombinant AREG or 200 ng/ml Gefitinib. Proliferation of CD4⁺ T cells was determined in different treatment groups control by CFSE analysis (A) and the results were analyzed statistically (B). *p < 0.05, ***p < 0.001